UCM 2 Story
Brain function is based on the rapid communication between neurons through neurotransmitter release. The goal of this group is to understand the mechanisms that modulate synaptic transmission. To this end, biochemical, molecular biology, genetics, electrophysiological and imaging tools are applied to preparations of synaptic terminals, cultured neurons and cerebral slices in order to obtain (i) the functional image of the vesicular recycling with fluorescent probes. Presynaptically, we currently study how presynaptic receptors such as β-adrenergic receptors and endocannabinoids CB1 receptors influence synaptic vesicle distribution and release competence through the recruitment of release machinery proteins (RIMs and Munc13s). Postsynaptically, we are interested in the retrograde modulation synaptic vesicle recycling by the NMDA/NO/cGMP signaling pathway. (ii) information on the modulation of glutamate and synaptic transmission by G protein coupled receptors. The goal is the study of the bidirectional control by mGlu7 receptors and the potentiating effect of β-adrenergic receptors. In addition, alterations in receptor function in a mouse model of fragile X syndrome will be established. (iii) In the last years we are also interested in the potential use of peritoneal dialysis and hemodialysis as a multitarget therapy to reduce the neurological caused by ictus by promoting the removal of glutamate from the interstitial space to blood.
Jose Sanchez-Prieto Borja, Doctorate in Pharmacy (Biochemistry, 1982). Postdoctoral formation in St Bartholomew´s Hospital (London, UK), Ninewells Hospital (Dundee, Scotland) and Cajal Institute, CSIC (Madrid). Principal investigator in 26 research projects (EU, Spain, regional government, private institutions). Author of 96 scientific publications and 169 congress communication. JS-PB has been invited to 34 conferences in International (24) and national (10) meetings. Member of the Editorial Committee of the Journal of Neurochemistry (2002-2007). Supervisor of 10 Doctoral Thesis and 2 more in progress. The Dr Sanchez-Prieto research work has been the study of the presynaptic mechanisms modulating glutamate release with focus on presynaptic receptors and the signaling pathways. More recently we participate in translational projects to understand the alterations of the function of presynaptic receptors in a mouse model of fragile X syndrome. We also participate in a project that uses the power peritoneal dialysis and hemodialysis to modify the relationship between plasmatic and cerebral glutamate to reduce the cerebral damage caused by a ischemic episode.
Magdalena Torres Molina, Doctor in Chemistry. Postdoctoral formación in postdoctoral en Centre de Neurochimie (Strasbourg, Francia), NIH (Bethesda, USA) and University of Virginia (Charlottesville, USA). She has published 70 scientific articles and presented more than 146 communications to Congress. She has been invited to 8 conferences (7 international and 1 national meetings). She has participate in the supervision of 7 Doctoral Thesis. Her research work has been the characterization of glucose and adenosine transporters and the role of NO-cGMP signaling in the regulation of synaptic transmission. More recently, she participates in translational projects with the aim at reducing the cerebral ischemic damage by accelerating brain glutamate clearance with peritoneal dialysis and hemodialysis. Colaborations: Dr. Ulrich Förstermann (Johannes Gutenberg Universität Mainz-Alemania); Dr. Edward Ziff (New York University, School of Medicine, New York, - USA) and Dr. Franz Hofmann, (Technische Universität, München-Germany). Ramón y Cajal and Juan de la Cierva programes evaluator (2006 and 2007). Expert panel member of the: PROGRAMA ACADEMIA ANECA, Health area since October 2009; Commission member: PROGRAMA VERIFICA DOCTORADO ANECA, Health area 2012- 2016 and PROGRAMA MONITOR from October 2014-February 2015.
Ricardo Martin Herranz, Doctor in Biochemistry (2010) with a research project related to the modulation of glutamate release by mGlu7 receptors. Postdoctoral formation with Dr. Brose from the Max Planck Institute for Experimental Medicine (Göttingen, Germany) to study conformational changes of mutant Munc13 proteins with BRET molecular cloning and with Dr A Araque (Instituto Cajal, Madrid) to study by means of Ca2+ imaging and cell recordings the existence of specific astrocyte-neuron networks of the direct and indirect pathways of basal ganglia and possible alterations of this communication in Parkinson disease. Currently we are interested in the bidirectional modulation of synaptic transmission by mGlu7 receptors in fragile X syndrome by means of intracelular recordings, electron microscopy and genetics. He has participated in 11 publications including J Biol Chem 2010, Science 2015, J Physiol 2018. He has been invited to 4 international conferences. He has participated in teaching: Master of Neuroscience, UAM, Madrid; master in Biochemistry Molecular Biology and Biomedicine, UCM, Madrid; master in neuroscience, UCM, Madrid; International CAJAL-ISN course: in the FENS course (Bordeaux, France). Juan de la Cierva Fellowship (2012-2014) and Olimpus Award for young investigators SENC, 2017.
Alberto Rampérez Martin, Degree in Biochemistry (2012) and Doctorate in Biochemsitry (2019). Collaboration Fellowship (2011-2012). Master Thesis with honors on “Intersynaptic vesicular traffic in rat cerebellar granule cells” supervised by Dr Magdalena Torres. Predoctoral fellowship FPI Program (2014-2017). Thesis on: the mechanism of endocytosis in rat cerebellar granule cells and the role of NO and cGMP signaling in RhoA/ROCK mediated vesicular recycling. Ultrastructure of the synapses under different stimulation protocols show differences between wild type and synapses lacking FMRP protein. He has participated in 8 national and 1 international meeting. 1 publication in J. Neurochemistry. 2 more manuscripts under revision.
Nuria Garcia Font, Degree in Pharmacy, 2013. Master in Neuroscience, 2014. Master Thesis on “Compounds with acetylcholinesterase inhibitory activity combined with neuroprotection and β amyloid peptide antiagregation activities. Supervides by Drs María Jesús Oset Gasque and José Luis Marco Contelles. Doctorate Thesis (Supervisors: Drs MJ Oset and J Sánchez-Prieto) “Changes in the modulation of glutamate release by mGluR7, CB1 and β adrenergic receptors in the fragile X syndrome”. Predoctoral Fellowship UCM Program. 2017. 4 publications and 2 more manuscripts in preparation. One dissemination publication.